Optimising immunohistochemistry (IHC) antibody dilution involves balancing staining intensity against background. The ideal working dilution depends on the antibody, tissue, target expression and staining protocol.
ABclonal presents four real staining examples across different tissues and targets, with reported working dilutions spanning 1:2,000 to 1:40,000. These examples demonstrate the importance of evaluating antibody performance under the specific conditions of an IHC experiment.
CD163 IHC staining — ABclonal A26411PM: view product details and recommended conditions
CD68 IHC staining — ABclonal A24386PM: view product details and recommended conditions
Occludin IHC staining — ABclonal A24601: view product details and recommended conditions
CD20 IHC staining — ABclonal A4893: view product details and recommended conditions
A higher dilution uses less primary antibody, but the highest usable dilution cannot be assumed to work for every tissue or protocol. For reliable results, use the manufacturer’s application data as a starting point and optimise staining intensity, specificity and background with appropriate controls.
Explore the ABclonal CD163, CD68, Occludin and CD20 antibodies above for their individual validation images and product information.
Genesearch is the Australian distributor for ABclonal research reagents. For product enquiries, availability or ordering, browse the Genesearch website or use the contact details or live chat available on the site.
For research use only. Staining performance and suitable working dilutions depend on experimental conditions.
